Biomarker Discovery:Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells.
Article Snippet: .. Generation and validation of M3-9-M with cre-inducible mCherry To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Plasmid Preparation:Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells.
Article Snippet: .. Generation and validation of M3-9-M with cre-inducible mCherry To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells
Article Snippet: .. To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Expressing:Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells.
Article Snippet: .. Generation and validation of M3-9-M with cre-inducible mCherry To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells
Article Snippet: .. To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Polymerase Chain Reaction:Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells.
Article Snippet: .. Generation and validation of M3-9-M with cre-inducible mCherry To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells
Article Snippet: .. To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Amplification:Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells.
Article Snippet: .. Generation and validation of M3-9-M with cre-inducible mCherry To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells
Article Snippet: .. To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Clone Assay:Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells.
Article Snippet: .. Generation and validation of M3-9-M with cre-inducible mCherry To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells
Article Snippet: .. To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Sequencing:Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells.
Article Snippet: .. Generation and validation of M3-9-M with cre-inducible mCherry To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells
Article Snippet: .. To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Modification:Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells.
Article Snippet: .. Generation and validation of M3-9-M with cre-inducible mCherry To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells
Article Snippet: .. To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Virus:Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells.
Article Snippet: .. Generation and validation of M3-9-M with cre-inducible mCherry To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells
Article Snippet: .. To generate the plasmid for constitutive mTagBFP2 expression and cre-inducible mCherry expression, an mTagBFP2 gene was PCR amplified from the mTagBFP2-pBAD plasmid (Addgene, 54572) and cloned upstream of a T2A sequence in a lentivirus expression vector (pLV-eGFP, Addgene, 36083), modified to be driven by the Murine Stem Cell Virus (MSCV) promoter. .. Next, a loxP-STOP-loxP sequence (PCR amplified from the pCAG-loxPSTOPloxP-ZsGreen plasmid, Addgene, 51269) was cloned downstream of the T2A, followed by the gene encoding mCherry.
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